RESUMEN
INTRODUCTION: Chronic recurrent cystitis (CRC) is a complex multifaceted problem of modern uroinfectology. OBJECTIVE: To study the immunological parameters of urine in patients with chronic recurrent cystitis depending on the etiological factor. MATERIALS AND METHODS: The prospective study included 71 patients aged 20-45 years who had previously been diagnosed with recurrent lower urinary tract infection: chronic recurrent cystitis (CRC) during an exacerbation period. Based on the results of bacteriological and PCR studies of urine, scraping of the urethra and vagina, depending on the dominant etiological factor, the patients were divided into three groups: group 1 (n=30) - with papillomavirus CRC (PVI-CRC), group 2 (n=30) - with bacterial CRC (B - CRC), group 3 (n=11) - with candida CRC (C - CRC). Analysis of the assessment of immunological parameters of urine was carried out using an enzyme-linked immunosorbent assay (ELISA-BEST). RESULTS: Based on the results of an immunological study of urine in the study groups, characteristic specific changes in the level of interleukins and interferons were identified, which made it possible to determine a protocol for the differential diagnosis of CRC. CONCLUSIONS: Our study shows the advisability of testing interleukins in urine (IL-1 beta, IL-6, IL-8); these indicators can serve as scoring criteria in the differential diagnosis of CRC of various origins. CONCLUSIONS: , it is reasonable to study the level of IFN-2b and IFN; when identifying the functional inferiority of the IFN system in women with CRC, correction of the IFN system is necessary.
Asunto(s)
Cistitis , Humanos , Femenino , Cistitis/orina , Cistitis/diagnóstico , Cistitis/inmunología , Adulto , Persona de Mediana Edad , Diagnóstico Diferencial , Enfermedad Crónica , Estudios Prospectivos , Recurrencia , Interleucinas/orina , Infecciones por Papillomavirus/orina , Infecciones por Papillomavirus/inmunología , Infecciones por Papillomavirus/diagnóstico , Adulto Joven , Interferones/orinaRESUMEN
Endogenous interferon was produced in animals in response to the administration of tobacco mosaic virus (TMV), tilorone and sodium nucleinate. The relationship between interferon production and the kind of inducer and the route of its administration was studied. TMV was completely innocuous for Macaca rhesus monkeys and mice and caused no untoward effects in humans upon peroral administration. TMV, tilorone and sodium nucleinate given per os exerted a marked protective effect in mice against tick-borne encephalitis, eastern and western equine encephalomyelitis and influenza virus infections.
Asunto(s)
Fluorenos/farmacología , Inductores de Interferón , Interferones/biosíntesis , Ácidos Nucleicos/farmacología , Tilorona/farmacología , Virus del Mosaico del Tabaco , Administración Oral , Animales , Evaluación Preclínica de Medicamentos , Encefalitis Transmitida por Garrapatas/prevención & control , Encefalomielitis Equina/prevención & control , Humanos , Gripe Humana/prevención & control , Inductores de Interferón/administración & dosificación , Interferones/orina , Ratones , Ácidos Nucleicos/administración & dosificación , Conejos , Tilorona/administración & dosificaciónRESUMEN
The effect of removal of sialic acid on the survival of rabbit serum and urinary interferon (IF) has been investigated in isolate, perfused rabbit liver preparations. In contrast with native IF, which may be already partially desialylated. IF freed of 80 to 90% of its sialic acid was rapidly cleared from the perfusate of normal livers, or livers pre-treated with actinomycin D. The results suggest that the mechanism of IF catabolism by the liver is similar to that reported for several other circulating glycoproteins.
Asunto(s)
Interferones/metabolismo , Hígado/metabolismo , Ácidos Siálicos/metabolismo , Animales , Membrana Celular/metabolismo , Dactinomicina/farmacología , Interferones/sangre , Interferones/orina , Hígado/efectos de los fármacos , Neuraminidasa/metabolismo , Perfusión , ConejosRESUMEN
Rabbit interferon has been extensively desialytated and its metabolic behaviour has been evaluated in the rabbit. The half-life of asialointerferon is significantly shorter than the native interferon and its urinary excretion becomes negligible. Moreover the rapid uptake of asialointerferon by the isolated and perfused rabbit liver, suggests a hitherto unsuspected catobolic pathway for this glycoprotein.
Asunto(s)
Interferones/metabolismo , Ácidos Siálicos/metabolismo , Animales , Interferones/sangre , Interferones/orina , Cinética , Hígado/metabolismo , Neuraminidasa , ConejosAsunto(s)
Líquidos Corporales/metabolismo , Interferones/metabolismo , Animales , Femenino , Humanos , Interferones/sangre , Interferones/orina , Riñón/metabolismo , Hígado/metabolismo , Intercambio Materno-Fetal , Tasa de Depuración Metabólica , Modelos Biológicos , Embarazo , Conejos , Distribución TisularRESUMEN
The distribution of homologous and heterologous interferon in rabbits after intratracheal administration was studied. The content of interferon in the lung tissue decreased 1 hour after injection. At 3 hours the interferon concentration in the lungs in some animals decreased 2-10-fold as compared with the initial. A considerable reduction in interferon content in the lungs was observed 24 hours post-injection. In the blood serum interferon was found in low titers 1, 3 and 6 hours after intratracheal injection. On the contrary, the amount of interferon in the urine increased beginning at 3-6 hours after injection and up to 24-48 hours.
